primary antibody hdac8 sheep Search Results


hdac8  (R&D Systems)
93
R&D Systems hdac8
Hdac8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hdac8 proteintech 17548 1 ap  (Proteintech)
93
Proteintech hdac8 proteintech 17548 1 ap
Hdac8 Proteintech 17548 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody hdac8 sheep  (R&D Systems)
90
R&D Systems primary antibody hdac8 sheep
Primary Antibody Hdac8 Sheep, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arl13b  (Proteintech)
96
Proteintech arl13b
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
Arl13b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/arl13b/product/Proteintech
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anti-h3k27me3 07-449  (Millipore)
90
Millipore anti-h3k27me3 07-449
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
Anti H3k27me3 07 449, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-hdac2 39934  (Active Motif)
90
Active Motif anti-hdac2 39934
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
Anti Hdac2 39934, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-hdac2  (Active Motif)
90
Active Motif anti-hdac2
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
Anti Hdac2, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nf-m  (GeneTex)
90
GeneTex nf-m
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
Nf M, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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f4/80  (GeneTex)
90
GeneTex f4/80
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
F4/80, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho c jun  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho c jun
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
Phospho C Jun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c jun  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc c jun
Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and <t>ARL13B.</t> Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).
C Jun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bcl2 antibody  (Proteintech)
96
Proteintech bcl2 antibody
Figure 4. SS induces cell cycle arrest and apoptosis in CRC cells. (A) Flow cytometry analysis to determine the cycle of SW620 cells after 4 h treatment in blank group, SS group (40 µM) and 5-FU group (0.7 mM). (B) Western blot analysis of CDK2 and p21 expression levels in SW620 cells after 24 h of incubation in the blank group, SS group (40 µM) and 5-FU group (0.7 mM). (C) Flow cytometry analysis to determine the apoptosis of SW620 cells in the blank group, SS group (40 µM) and 5-FU group (0.7 mM) after 24 h of culture. (D) Western blot analysis of the expression levels of cleave Caspase-3, <t>Bcl2</t> and Cyt C in SW620 cells after 24 h of culture in the blank group, SS group (40 µM) and 5-FU group (0.7 mM). All experiments were performed three times independently. * p < 0.05, ** p < 0.01, *** p < 0.001.
Bcl2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and ARL13B. Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).

Journal: Biology Open

Article Title: HDAC3 and HDAC8 are required for cilia assembly and elongation

doi: 10.1242/bio.043828

Figure Lengend Snippet: Involvement of the class I HDACs in the cilia formation. (A) RPE1 cells were pre-treated with TSA for 12 h and cultured in serum-deprived medium. Forty-eight hours later, the cells were immunostained with antibodies specific to acetylated α-tubulin and ARL13B. Scale bar: 2 μm. (B) The number of cells with cilia was determined. Greater than 100 cells per experimental group were counted in three independent experiments. (C) Ciliary length was measured. Greater than 30 cilia per experimental group were measured in three independent experiments. Values are means and s.e.m. Statistical significance was analyzed using one-way ANOVA and is indicated by *( P <0.05).

Article Snippet: The primary antibodies used were specific to HDAC1 (Abcam, ab31263), HDAC2 (Abcam, ab12169), HDAC3 (Abcam, ab7030), HDAC8 (Proteintech, 17548-1-AP), HDAC6 (Abgent, AP1106a), acetylated α-tubulin (Cell Signaling Technology; 5335S, Sigma-Aldrich; T6793), ARL13B (Proteintech, 17711-1-AP), Flag (Sigma-Aldrich, F3165), or GAPDH (Ambion, AM4300).

Techniques: Cell Culture

Figure 4. SS induces cell cycle arrest and apoptosis in CRC cells. (A) Flow cytometry analysis to determine the cycle of SW620 cells after 4 h treatment in blank group, SS group (40 µM) and 5-FU group (0.7 mM). (B) Western blot analysis of CDK2 and p21 expression levels in SW620 cells after 24 h of incubation in the blank group, SS group (40 µM) and 5-FU group (0.7 mM). (C) Flow cytometry analysis to determine the apoptosis of SW620 cells in the blank group, SS group (40 µM) and 5-FU group (0.7 mM) after 24 h of culture. (D) Western blot analysis of the expression levels of cleave Caspase-3, Bcl2 and Cyt C in SW620 cells after 24 h of culture in the blank group, SS group (40 µM) and 5-FU group (0.7 mM). All experiments were performed three times independently. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Molecules (Basel, Switzerland)

Article Title: Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway.

doi: 10.3390/molecules28186649

Figure Lengend Snippet: Figure 4. SS induces cell cycle arrest and apoptosis in CRC cells. (A) Flow cytometry analysis to determine the cycle of SW620 cells after 4 h treatment in blank group, SS group (40 µM) and 5-FU group (0.7 mM). (B) Western blot analysis of CDK2 and p21 expression levels in SW620 cells after 24 h of incubation in the blank group, SS group (40 µM) and 5-FU group (0.7 mM). (C) Flow cytometry analysis to determine the apoptosis of SW620 cells in the blank group, SS group (40 µM) and 5-FU group (0.7 mM) after 24 h of culture. (D) Western blot analysis of the expression levels of cleave Caspase-3, Bcl2 and Cyt C in SW620 cells after 24 h of culture in the blank group, SS group (40 µM) and 5-FU group (0.7 mM). All experiments were performed three times independently. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Bcl2 antibody (Cat No: 68103-1-Ig), Caspase3 antibody (Cat No: 66470-2-Ig), Cytochrome C (Cyt C) antibody (Cat No: 10993-1-AP), p53 antibody (Cat No: 60283-2-Ig), p21 antibody (Cat No: 10355-1-AP), CDK2 antibody (Cat No: 10122-1-AP), HDAC1 antibody (Cat No: 66085-1-Ig), HDAC2 antibody (Cat No: 67165-1-Ig), HDAC3 antibody (Cat No: 67151-1-Ig) and HDAC8 antibody (Cat No: 17548-1 AP) were purchased from Proteintech, Wuhan, China.

Techniques: Flow Cytometry, Western Blot, Expressing, Incubation

Figure 7. SS acts through class I HDAC-induced p53 acetylation. (A) IHC assessment of the effect of SS on Ki-67 expression levels in CRC cell nude mice subcutaneously transplanted with tumors. (B) Weight–time curves were plotted based on the body weight of nude mice measured during SS administration. (C) Tumor appearance and mean weight of tumors in each drug group. (D) HE staining to detect the inhibitory effect of SS on subcutaneously transplanted tumors in CRC cell nude mice. (E) IHC detection of SS-induced apoptosis of tumor cells. (F) Western blot analysis of the effects of acetylation of H3K27, H3K18, H3K9 and H4 in CRC cell nude mice subcutaneously transplanted tumors in the blank group, 5-FU group (25 mg/kg), SS group (50 mg/kg), Ent group (25 mg/kg) and SS (50 mg/kg) + Ent (25 mg/kg) group. (G) Protein blot analysis to detect the protein expression of class I HDAC in SS, Ent and SS + Ent-treated CRC cells. (H) Western blot analysis of the expression levels of cleave Caspase-3 and Bcl2 in SW620 cells 24 h after drug administration. (I) Western blot analysis of CDK2 and p21 expression levels in SW620 cells 24 h after drug administration in culture. All experiments were performed three times independently. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Molecules (Basel, Switzerland)

Article Title: Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway.

doi: 10.3390/molecules28186649

Figure Lengend Snippet: Figure 7. SS acts through class I HDAC-induced p53 acetylation. (A) IHC assessment of the effect of SS on Ki-67 expression levels in CRC cell nude mice subcutaneously transplanted with tumors. (B) Weight–time curves were plotted based on the body weight of nude mice measured during SS administration. (C) Tumor appearance and mean weight of tumors in each drug group. (D) HE staining to detect the inhibitory effect of SS on subcutaneously transplanted tumors in CRC cell nude mice. (E) IHC detection of SS-induced apoptosis of tumor cells. (F) Western blot analysis of the effects of acetylation of H3K27, H3K18, H3K9 and H4 in CRC cell nude mice subcutaneously transplanted tumors in the blank group, 5-FU group (25 mg/kg), SS group (50 mg/kg), Ent group (25 mg/kg) and SS (50 mg/kg) + Ent (25 mg/kg) group. (G) Protein blot analysis to detect the protein expression of class I HDAC in SS, Ent and SS + Ent-treated CRC cells. (H) Western blot analysis of the expression levels of cleave Caspase-3 and Bcl2 in SW620 cells 24 h after drug administration. (I) Western blot analysis of CDK2 and p21 expression levels in SW620 cells 24 h after drug administration in culture. All experiments were performed three times independently. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Bcl2 antibody (Cat No: 68103-1-Ig), Caspase3 antibody (Cat No: 66470-2-Ig), Cytochrome C (Cyt C) antibody (Cat No: 10993-1-AP), p53 antibody (Cat No: 60283-2-Ig), p21 antibody (Cat No: 10355-1-AP), CDK2 antibody (Cat No: 10122-1-AP), HDAC1 antibody (Cat No: 66085-1-Ig), HDAC2 antibody (Cat No: 67165-1-Ig), HDAC3 antibody (Cat No: 67151-1-Ig) and HDAC8 antibody (Cat No: 17548-1 AP) were purchased from Proteintech, Wuhan, China.

Techniques: Expressing, Staining, Western Blot